be001 1 anti mouse cd28 bioxcell Search Results


cd28  (Sino Biological)
94
Sino Biological cd28
Cd28, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
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anti cd28  (Bio X Cell)
97
Bio X Cell anti cd28
Anti Cd28, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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405303 mouse anti human cd3 okt3 bioxcell be0001 2 mouse anti human cd28  (Bio X Cell)
97
Bio X Cell 405303 mouse anti human cd3 okt3 bioxcell be0001 2 mouse anti human cd28
Fig. 1. Characterization of the hydrogel system for T cell activation. (A) Schematic of antibody coated hydrogels as T cell activation platform. Poly acrylamide gels were polymerized with streptavidin- acrylamide, then coated with biotinylated Goat-anti- mouse and secondary activating antibodies to <t>CD3</t> and <t>CD28.</t> Mixed CD4+/CD8+ polyclonal primary human T cells were stimulated on activating sub strates. (B) Mechanical testing via indentation indi cate hydrogels with varying ratios of acrylamide and bis-acrylamide have Young’s modulus between 5 and 110 kPa (Data are mean ± s.d., n = 4 gels for 5 kPa, n = 7 gels for all other formulations). (C) Confocal microscopy imaging of fluorescent antibodies coated on hydrogels indicate attachment of antibodies to hydrogel surface. (D) Streptavidin-acrylamide con centrations were varied to obtain similar coating of antibodies on hydrogels, as verified by fluorescence intensities on the surface of gels. (n = 3 gels for each formulation).
405303 Mouse Anti Human Cd3 Okt3 Bioxcell Be0001 2 Mouse Anti Human Cd28, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/be001+1+anti+mouse+cd28+bioxcell/pm33878536-39-12-16?v=Bio+X+Cell
Average 97 stars, based on 1 article reviews
405303 mouse anti human cd3 okt3 bioxcell be0001 2 mouse anti human cd28 - by Bioz Stars, 2026-08
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soluble anti cd28  (Bio X Cell)
93
Bio X Cell soluble anti cd28
Fig. 1. Characterization of the hydrogel system for T cell activation. (A) Schematic of antibody coated hydrogels as T cell activation platform. Poly acrylamide gels were polymerized with streptavidin- acrylamide, then coated with biotinylated Goat-anti- mouse and secondary activating antibodies to <t>CD3</t> and <t>CD28.</t> Mixed CD4+/CD8+ polyclonal primary human T cells were stimulated on activating sub strates. (B) Mechanical testing via indentation indi cate hydrogels with varying ratios of acrylamide and bis-acrylamide have Young’s modulus between 5 and 110 kPa (Data are mean ± s.d., n = 4 gels for 5 kPa, n = 7 gels for all other formulations). (C) Confocal microscopy imaging of fluorescent antibodies coated on hydrogels indicate attachment of antibodies to hydrogel surface. (D) Streptavidin-acrylamide con centrations were varied to obtain similar coating of antibodies on hydrogels, as verified by fluorescence intensities on the surface of gels. (n = 3 gels for each formulation).
Soluble Anti Cd28, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/be001+1+anti+mouse+cd28+bioxcell/pmc07823996-171-29-36?v=Bio+X+Cell
Average 93 stars, based on 1 article reviews
soluble anti cd28 - by Bioz Stars, 2026-08
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anti mouse cd28  (Bio X Cell)
94
Bio X Cell anti mouse cd28
Fig. 1. Characterization of the hydrogel system for T cell activation. (A) Schematic of antibody coated hydrogels as T cell activation platform. Poly acrylamide gels were polymerized with streptavidin- acrylamide, then coated with biotinylated Goat-anti- mouse and secondary activating antibodies to <t>CD3</t> and <t>CD28.</t> Mixed CD4+/CD8+ polyclonal primary human T cells were stimulated on activating sub strates. (B) Mechanical testing via indentation indi cate hydrogels with varying ratios of acrylamide and bis-acrylamide have Young’s modulus between 5 and 110 kPa (Data are mean ± s.d., n = 4 gels for 5 kPa, n = 7 gels for all other formulations). (C) Confocal microscopy imaging of fluorescent antibodies coated on hydrogels indicate attachment of antibodies to hydrogel surface. (D) Streptavidin-acrylamide con centrations were varied to obtain similar coating of antibodies on hydrogels, as verified by fluorescence intensities on the surface of gels. (n = 3 gels for each formulation).
Anti Mouse Cd28, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/be001+1+anti+mouse+cd28+bioxcell/pmc10205730__41467_2023_38707_MOESM3_ESM-16-130-132?v=Bio+X+Cell
Average 94 stars, based on 1 article reviews
anti mouse cd28 - by Bioz Stars, 2026-08
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anti human cd3  (Bio X Cell)
95
Bio X Cell anti human cd3
Fig. 1. Characterization of the hydrogel system for T cell activation. (A) Schematic of antibody coated hydrogels as T cell activation platform. Poly acrylamide gels were polymerized with streptavidin- acrylamide, then coated with biotinylated Goat-anti- mouse and secondary activating antibodies to <t>CD3</t> and <t>CD28.</t> Mixed CD4+/CD8+ polyclonal primary human T cells were stimulated on activating sub strates. (B) Mechanical testing via indentation indi cate hydrogels with varying ratios of acrylamide and bis-acrylamide have Young’s modulus between 5 and 110 kPa (Data are mean ± s.d., n = 4 gels for 5 kPa, n = 7 gels for all other formulations). (C) Confocal microscopy imaging of fluorescent antibodies coated on hydrogels indicate attachment of antibodies to hydrogel surface. (D) Streptavidin-acrylamide con centrations were varied to obtain similar coating of antibodies on hydrogels, as verified by fluorescence intensities on the surface of gels. (n = 3 gels for each formulation).
Anti Human Cd3, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 95 stars, based on 1 article reviews
anti human cd3 - by Bioz Stars, 2026-08
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be001 1 anti mouse cd28 bioxcell  (Bio X Cell)
94
Bio X Cell be001 1 anti mouse cd28 bioxcell
Fig. 1. Characterization of the hydrogel system for T cell activation. (A) Schematic of antibody coated hydrogels as T cell activation platform. Poly acrylamide gels were polymerized with streptavidin- acrylamide, then coated with biotinylated Goat-anti- mouse and secondary activating antibodies to <t>CD3</t> and <t>CD28.</t> Mixed CD4+/CD8+ polyclonal primary human T cells were stimulated on activating sub strates. (B) Mechanical testing via indentation indi cate hydrogels with varying ratios of acrylamide and bis-acrylamide have Young’s modulus between 5 and 110 kPa (Data are mean ± s.d., n = 4 gels for 5 kPa, n = 7 gels for all other formulations). (C) Confocal microscopy imaging of fluorescent antibodies coated on hydrogels indicate attachment of antibodies to hydrogel surface. (D) Streptavidin-acrylamide con centrations were varied to obtain similar coating of antibodies on hydrogels, as verified by fluorescence intensities on the surface of gels. (n = 3 gels for each formulation).
Be001 1 Anti Mouse Cd28 Bioxcell, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/be001+1+anti+mouse+cd28+bioxcell/10__1158_slash_0008___5472__can___21___4052-95-6-9?v=Bio+X+Cell
Average 94 stars, based on 1 article reviews
be001 1 anti mouse cd28 bioxcell - by Bioz Stars, 2026-08
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cd3 epsilon antibody  (NSJ Bioreagents)
99
NSJ Bioreagents cd3 epsilon antibody
Fig. 1. Characterization of the hydrogel system for T cell activation. (A) Schematic of antibody coated hydrogels as T cell activation platform. Poly acrylamide gels were polymerized with streptavidin- acrylamide, then coated with biotinylated Goat-anti- mouse and secondary activating antibodies to <t>CD3</t> and <t>CD28.</t> Mixed CD4+/CD8+ polyclonal primary human T cells were stimulated on activating sub strates. (B) Mechanical testing via indentation indi cate hydrogels with varying ratios of acrylamide and bis-acrylamide have Young’s modulus between 5 and 110 kPa (Data are mean ± s.d., n = 4 gels for 5 kPa, n = 7 gels for all other formulations). (C) Confocal microscopy imaging of fluorescent antibodies coated on hydrogels indicate attachment of antibodies to hydrogel surface. (D) Streptavidin-acrylamide con centrations were varied to obtain similar coating of antibodies on hydrogels, as verified by fluorescence intensities on the surface of gels. (n = 3 gels for each formulation).
Cd3 Epsilon Antibody, supplied by NSJ Bioreagents, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd3 epsilon antibody - by Bioz Stars, 2026-08
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cd3e, mouse(145-2c11)  (Biotium)
99
Biotium cd3e, mouse(145-2c11)
Fig. 1. Characterization of the hydrogel system for T cell activation. (A) Schematic of antibody coated hydrogels as T cell activation platform. Poly acrylamide gels were polymerized with streptavidin- acrylamide, then coated with biotinylated Goat-anti- mouse and secondary activating antibodies to <t>CD3</t> and <t>CD28.</t> Mixed CD4+/CD8+ polyclonal primary human T cells were stimulated on activating sub strates. (B) Mechanical testing via indentation indi cate hydrogels with varying ratios of acrylamide and bis-acrylamide have Young’s modulus between 5 and 110 kPa (Data are mean ± s.d., n = 4 gels for 5 kPa, n = 7 gels for all other formulations). (C) Confocal microscopy imaging of fluorescent antibodies coated on hydrogels indicate attachment of antibodies to hydrogel surface. (D) Streptavidin-acrylamide con centrations were varied to obtain similar coating of antibodies on hydrogels, as verified by fluorescence intensities on the surface of gels. (n = 3 gels for each formulation).
Cd3e, Mouse(145 2c11), supplied by Biotium, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/be001+1+anti+mouse+cd28+bioxcell/custom%40bnc042013-100%40pmc10120060__pnas__2210047120__sapp?v=Biotium
Average 99 stars, based on 1 article reviews
cd3e, mouse(145-2c11) - by Bioz Stars, 2026-08
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be001  (Bio X Cell)
95
Bio X Cell be001
Fig. 1. Characterization of the hydrogel system for T cell activation. (A) Schematic of antibody coated hydrogels as T cell activation platform. Poly acrylamide gels were polymerized with streptavidin- acrylamide, then coated with biotinylated Goat-anti- mouse and secondary activating antibodies to <t>CD3</t> and <t>CD28.</t> Mixed CD4+/CD8+ polyclonal primary human T cells were stimulated on activating sub strates. (B) Mechanical testing via indentation indi cate hydrogels with varying ratios of acrylamide and bis-acrylamide have Young’s modulus between 5 and 110 kPa (Data are mean ± s.d., n = 4 gels for 5 kPa, n = 7 gels for all other formulations). (C) Confocal microscopy imaging of fluorescent antibodies coated on hydrogels indicate attachment of antibodies to hydrogel surface. (D) Streptavidin-acrylamide con centrations were varied to obtain similar coating of antibodies on hydrogels, as verified by fluorescence intensities on the surface of gels. (n = 3 gels for each formulation).
Be001, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/be001+1+anti+mouse+cd28+bioxcell/10__1158_slash_0008___5472__can___21___4052-95-20-23?v=Bio+X+Cell
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invivomab polyclonal armenian hamster igg  (Bio X Cell)
96
Bio X Cell invivomab polyclonal armenian hamster igg
Fig. 1. Characterization of the hydrogel system for T cell activation. (A) Schematic of antibody coated hydrogels as T cell activation platform. Poly acrylamide gels were polymerized with streptavidin- acrylamide, then coated with biotinylated Goat-anti- mouse and secondary activating antibodies to <t>CD3</t> and <t>CD28.</t> Mixed CD4+/CD8+ polyclonal primary human T cells were stimulated on activating sub strates. (B) Mechanical testing via indentation indi cate hydrogels with varying ratios of acrylamide and bis-acrylamide have Young’s modulus between 5 and 110 kPa (Data are mean ± s.d., n = 4 gels for 5 kPa, n = 7 gels for all other formulations). (C) Confocal microscopy imaging of fluorescent antibodies coated on hydrogels indicate attachment of antibodies to hydrogel surface. (D) Streptavidin-acrylamide con centrations were varied to obtain similar coating of antibodies on hydrogels, as verified by fluorescence intensities on the surface of gels. (n = 3 gels for each formulation).
Invivomab Polyclonal Armenian Hamster Igg, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Fig. 1. Characterization of the hydrogel system for T cell activation. (A) Schematic of antibody coated hydrogels as T cell activation platform. Poly acrylamide gels were polymerized with streptavidin- acrylamide, then coated with biotinylated Goat-anti- mouse and secondary activating antibodies to CD3 and CD28. Mixed CD4+/CD8+ polyclonal primary human T cells were stimulated on activating sub strates. (B) Mechanical testing via indentation indi cate hydrogels with varying ratios of acrylamide and bis-acrylamide have Young’s modulus between 5 and 110 kPa (Data are mean ± s.d., n = 4 gels for 5 kPa, n = 7 gels for all other formulations). (C) Confocal microscopy imaging of fluorescent antibodies coated on hydrogels indicate attachment of antibodies to hydrogel surface. (D) Streptavidin-acrylamide con centrations were varied to obtain similar coating of antibodies on hydrogels, as verified by fluorescence intensities on the surface of gels. (n = 3 gels for each formulation).

Journal: Biomaterials

Article Title: Biphasic response of T cell activation to substrate stiffness.

doi: 10.1016/j.biomaterials.2021.120797

Figure Lengend Snippet: Fig. 1. Characterization of the hydrogel system for T cell activation. (A) Schematic of antibody coated hydrogels as T cell activation platform. Poly acrylamide gels were polymerized with streptavidin- acrylamide, then coated with biotinylated Goat-anti- mouse and secondary activating antibodies to CD3 and CD28. Mixed CD4+/CD8+ polyclonal primary human T cells were stimulated on activating sub strates. (B) Mechanical testing via indentation indi cate hydrogels with varying ratios of acrylamide and bis-acrylamide have Young’s modulus between 5 and 110 kPa (Data are mean ± s.d., n = 4 gels for 5 kPa, n = 7 gels for all other formulations). (C) Confocal microscopy imaging of fluorescent antibodies coated on hydrogels indicate attachment of antibodies to hydrogel surface. (D) Streptavidin-acrylamide con centrations were varied to obtain similar coating of antibodies on hydrogels, as verified by fluorescence intensities on the surface of gels. (n = 3 gels for each formulation).

Article Snippet: Key resources table REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Biotinylated Goat-anti-mouse BioLegend 405303 Mouse-anti-human CD3 (OKT3) BioXCell BE0001-2 Mouse-anti-human CD28 (9.3) BioXCell BE0248 Biological Samples Leukopack New York Blood Center Chemicals, Peptides, and Recombinant Proteins Acrylamide (40%) Sigma 01697 Bis-acrylamide (2%) Fisher BP1404-250 Ammonium persulfate (APS) Sigma A3678 Tetramethylethylenediamine (TEMED) Sigma T7024 Streptavidin-acrylamide Fisher S21379 (3-Aminopropyl)triethoxysilane (APTES) Sigma 440140 Glutaraldehyde Sigma G7776 Alexa-fluor 488 Thermo A20000 Blebbistatin Sigma 203391 Prolong Diamond ThermoFisher P36961 Qiazol Qiagen 79306 Critical Commercial Assays Human IL-2 Secretion kit (APC) Miltenyi Biotec 130-090-763 RosetteSep Human T cell Enrichment Kit Stemcell Technologies 15061 CellTrace CFSE Dilution Assay ThermoFisher C34554 CellVue Jade ThermoFisher 88-0876-16 Software and Algorithms FlowJo 7.6 FlowJo LLC FCS Express V6 De Novo Software Graphpad Prism 7 Graphpad Software Kallisto Bray et al.

Techniques: Activation Assay, Confocal Microscopy, Imaging, Fluorescence, Formulation